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The T cell receptor (TCR) complex was purified from an effector T cell hybridoma and analyzed for its ability to mediate cell lysis. The TCR complex was found to require peptide antigen for cell lysis, and the functional activity of the TCR could not be replaced by the addition of purified proteins or isolated antibodies. Peptide binding was dependent on the monoclonal antibody specificity, and not on the epitope bound, since a panel of peptide analogs bearing one or two substitutions at a putative H-Y determinant at position 1 and one alanine substitution at position 2 bound equally well as the parent peptide. These results demonstrate that peptide antigen must be bound by the TCR in order to mediate a response. The CD3 protein molecules were also shown to be necessary to induce cell lysis, since addition of an anti-CD3 antibody alone was not sufficient to cause lysis. Overall, these results indicate that a cytotoxic T lymphocyte (CTL) response requires recognition of a peptide antigen by the TCR.Q:
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